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PurposeExpresses V5- and SBP-tagged hnRNPC1 in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 64920 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4229
- Total vector size (bp) 5111
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Modifications to backboneexchanged the EGFP tag by a V5- SBP-tag and introduced a T7 promoter
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehnRNPC1
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SpeciesH. sapiens (human)
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Insert Size (bp)882
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GenBank IDNM_001077443.1
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Entrez GeneHNRNPC (a.k.a. C1, C2, HNRNP, HNRPC, SNRPC)
- Promoter CMV
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Tag
/ Fusion Protein
- V5 and SBP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT7-V5-SBP-C1-HshnRNPC1 was a gift from Elisa Izaurralde (Addgene plasmid # 64920 ; http://n2t.net/addgene:64920 ; RRID:Addgene_64920)