pT7-V5-SBP-C1-HsRRP6
(Plasmid
#64916)
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Purposeexpresses V5- and SBP-tagged RRP6 in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 64916 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4213
- Total vector size (bp) 6871
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Modifications to backboneexchanged EGFP-Tag by a V5-SBP tag and introduced a T7 promoter
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRRP6
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Alt nameEXOSC10
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Alt namePMSCL
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SpeciesH. sapiens (human)
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Insert Size (bp)2658
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GenBank IDNM_001001998
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Entrez GeneEXOSC10 (a.k.a. PM-Scl, PM/Scl-100, PMSCL, PMSCL2, RRP6, Rrp6p, p2, p3, p4)
- Promoter CMV
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Tag
/ Fusion Protein
- V5 and SBP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 forw (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT7-V5-SBP-C1-HsRRP6 was a gift from Elisa Izaurralde (Addgene plasmid # 64916 ; http://n2t.net/addgene:64916 ; RRID:Addgene_64916)