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Addgene

pHL 2067
(Plasmid #64910)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64910 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ColE (from pZ system) + AmpR
  • Backbone size (bp) 3500
  • Vector type
    Bacterial Expression, Synthetic Biology ; E. coli

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    cfp is from Tsein Lab, psu is from pNL150 which was generously provided by Dr Richard Calendar (UC Berkeley), extra 5' UTR is PCR synthesized as described by A. Levin-Karp, U. Barenholz, T. Bareia, M. Dayagi, L. Zelcbuch, N. Antonovsky, E. Noor, R. Milo, Quantifying Translational Coupling in E. coli Synthetic Operons Using RBS Modulation and Fluorescent Reporters, ACS Synth Biol, 2 (2013)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHL 2067 was a gift from Han Lim (Addgene plasmid # 64910 ; http://n2t.net/addgene:64910 ; RRID:Addgene_64910)

  • For your References section:

    Quantitative characterization of gene regulation by Rho dependent transcription termination. Hussein R, Lee TY, Lim HN. Biochim Biophys Acta. 2015 May 14;1849(8):940-954. doi: 10.1016/j.bbagrm.2015.05.003. 10.1016/j.bbagrm.2015.05.003 PubMed 25982507
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