pHL 2057
(Plasmid
#64909)
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Purpose(Empty Backbone) AmpR + ColE1 + PLtetO-1::RBS(st7):cro::cII (31 basepairs)::T1 terminator + PLlacO-1::RBS(st7):psu::T1 terminator
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64909 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneColE (from pZ system) + AmpR
- Backbone size (bp) 3000
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Vector typeBacterial Expression, Synthetic Biology ; E. coli
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Cloning Information
- 5′ sequencing primer CTCATGA GCGGATACAT ATTTGAA (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bycro was PCR amplified from a Lambda DNA ladder/HindIII (Promega, Madison, WI, USA), psu is from pNL150 which was generously provided by Dr Richard Calendar (UC Berkeley)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHL 2057 was a gift from Han Lim (Addgene plasmid # 64909 ; http://n2t.net/addgene:64909 ; RRID:Addgene_64909) -
For your References section:
Quantitative characterization of gene regulation by Rho dependent transcription termination. Hussein R, Lee TY, Lim HN. Biochim Biophys Acta. 2015 May 14;1849(8):940-954. doi: 10.1016/j.bbagrm.2015.05.003. 10.1016/j.bbagrm.2015.05.003 PubMed 25982507
Map uploaded by the depositor.
