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Addgene

pHL 2057
(Plasmid #64909)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64909 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    ColE (from pZ system) + AmpR
  • Backbone size (bp) 3000
  • Vector type
    Bacterial Expression, Synthetic Biology ; E. coli

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    cro was PCR amplified from a Lambda DNA ladder/HindIII (Promega, Madison, WI, USA), psu is from pNL150 which was generously provided by Dr Richard Calendar (UC Berkeley)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHL 2057 was a gift from Han Lim (Addgene plasmid # 64909 ; http://n2t.net/addgene:64909 ; RRID:Addgene_64909)
  • For your References section:

    Quantitative characterization of gene regulation by Rho dependent transcription termination. Hussein R, Lee TY, Lim HN. Biochim Biophys Acta. 2015 May 14;1849(8):940-954. doi: 10.1016/j.bbagrm.2015.05.003. 10.1016/j.bbagrm.2015.05.003 PubMed 25982507