pBabe-Sox10
(Plasmid
#64890)
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PurposeRetroviral expression of murine Sox10
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 64890 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBabe
- Backbone size w/o insert (bp) 5100
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Vector typeRetroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesox10
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SpeciesM. musculus (mouse)
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GenBank ID20665
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Entrez GeneSox10 (a.k.a. Dom, Sox21, gt)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer pBABE5
- 3′ sequencing primer pBABE3 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr. Pavan, NIH, Bethesda, MD
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBabe-Sox10 was a gift from Ivana de la Serna (Addgene plasmid # 64890 ; http://n2t.net/addgene:64890 ; RRID:Addgene_64890) -
For your References section:
SWI/SNF enzymes promote SOX10- mediated activation of myelin gene expression. Marathe HG, Mehta G, Zhang X, Datar I, Mehrotra A, Yeung KC, de la Serna IL. PLoS One. 2013 Jul 16;8(7):e69037. doi: 10.1371/journal.pone.0069037. Print 2013. PONE-D-13-14967 [pii] PubMed 23874858