pBabe-MITF
(Plasmid #64888)

• Purpose: Retroviral expression of murine MITF
• Depositing Lab: Ivana de la Serna
• Publication: de la Serna et al J Biol Chem. 2006 Jul 21;281(29):20233-41. Epub 2006 Apr 28.

Backbone

• Vector backbone: pBABE-puro
• Backbone size w/o insert (bp): 5100
• Vector type: Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MITF
• Alt name: Microphthalmia-Associated Transcription Factor
• Species: M. musculus (mouse)
• Insert Size (bp): 1900
• GenBank ID: XM_006505695.2 XP_006505758.1
• Entrez Gene: Mitf (a.k.a. BCC2, Bhlhe32, Gsfbcc2, Vitiligo, Wh, bw, mi, vit)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: pBABE5
• 3′ sequencing primer: pBABE3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Steingrimsson, University of Iceland
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains the alternatively spliced MITF (-) isoform.