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Addgene

POLN delP_pDEST17
(Plasmid #64886)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64886 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST17
  • Backbone manufacturer
    Invitrogen
  • Total vector size (bp) 7387
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    POLN
  • Species
    H. sapiens (human)
  • Mutation
    with a short truncation of the C-terminal proline-rich tail
  • Entrez Gene
    POLN (a.k.a. POL4P)
  • Promoter T7
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • Flag (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    POLN delP_pDEST17 was a gift from Richard Wood (Addgene plasmid # 64886 ; http://n2t.net/addgene:64886 ; RRID:Addgene_64886)

  • For your References section:

    Human DNA polymerase N (POLN) is a low fidelity enzyme capable of error-free bypass of 5S-thymine glycol. Takata K, Shimizu T, Iwai S, Wood RD. J Biol Chem. 2006 Aug 18;281(33):23445-55. Epub 2006 Jun 19. 10.1074/jbc.M604317200 PubMed 16787914
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