pCDNA3.1_CoumRS
(Plasmid
#64882)
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PurposeExpresses Coumarinyl- tRNA synthetase
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64882 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCDNA3.1
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 6664
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCoum_RS
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Alt nameAminoacyl tRNA Synthetase
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SpeciesSynthetic; E.Coli
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Insert Size (bp)1299
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH-R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid is also associated with the following publication: https://elifesciences.org/articles/28626
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3.1_CoumRS was a gift from Sebastián Brauchi (Addgene plasmid # 64882 ; http://n2t.net/addgene:64882 ; RRID:Addgene_64882) -
For your References section:
A Rationally Designed Aminoacyl-tRNA Synthetase for Genetically Encoded Fluorescent Amino Acids. Steinberg X, Galpin J, Nasir G, Sepulveda-Ugarte J, Sepulveda R, Gonzalez-Nilo F, Islas LD, Ahern CA, Brauchi SE. bioRxiv, 10.1101/065227 10.1101/065227
Map uploaded by the depositor.
