pEGFP_TRPM8
(Plasmid #64879)

• Purpose: Expresses Ion Channel TRPM8 fused to eGFP in mammalian cells
• Depositing Lab: Sebastián Brauchi
• Publication: Toro et al J Neurosci. 2015 Jan 14;35(2):571-82. doi: 10.1523/JNEUROSCI.3820-13.2015.

Backbone

• Vector backbone: pEGFP_C3
• Backbone size w/o insert (bp): 4727
• Total vector size (bp): 8330

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Transient receptor potential cation channel subfamily M member 8
• Alt name: TRPM8
• Species: R. norvegicus (rat)
• Insert Size (bp): 3315
• GenBank ID: NM_134371.2
• Entrez Gene: Trpm8 (a.k.a. CMR1)
• Tag / Fusion Protein:
  • eGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XmaI (not destroyed)
• 5′ sequencing primer: CMV_F

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry