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JT113-pETDuet1-(R)-hREV3L
(Plasmid #64872)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64872 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETDuet-1
  • Backbone manufacturer
    EMD
  • Total vector size (bp) 14616
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    REV3L
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    9393
  • Entrez Gene
    REV3L (a.k.a. POLZ, REV3)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    JT113-pETDuet1-(R)-hREV3L was a gift from Richard Wood (Addgene plasmid # 64872 ; http://n2t.net/addgene:64872 ; RRID:Addgene_64872)

  • For your References section:

    REV7 is essential for DNA damage tolerance via two REV3L binding sites in mammalian DNA polymerase zeta. Tomida J, Takata K, Lange SS, Schibler AC, Yousefzadeh MJ, Bhetawal S, Dent SY, Wood RD. Nucleic Acids Res. 2015 Jan;43(2):1000-11. doi: 10.1093/nar/gku1385. Epub 2015 Jan 7. 10.1093/nar/gku1385 PubMed 25567983
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