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Addgene

pT7-V5-SBP-C1-HsRRP41
(Plasmid #64871)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64871 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4230
  • Total vector size (bp) 4966
  • Modifications to backbone
    exchanged EGFP-tag by a V5-SBP tag and introduced a T7 promoter
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RRP41
  • Alt name
    EXOSC4
  • Alt name
    Ski6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    738
  • GenBank ID
    NM_019037
  • Entrez Gene
    EXOSC4 (a.k.a. RRP41, RRP41A, Rrp41p, SKI6, Ski6p, hRrp41p, p12A)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5 and SBP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7-V5-SBP-C1-HsRRP41 was a gift from Elisa Izaurralde (Addgene plasmid # 64871 ; http://n2t.net/addgene:64871 ; RRID:Addgene_64871)
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