pRSET-A2-77p-Venus
(Plasmid #64856)

• Purpose: Expresses aa 1594-1670 of A2 Domain of VWF protein, TEV cleavage site and Venus followed by 7X His tag
• Depositing Lab: Sriram Neelamegham
• Publication: Gogia et al PLoS One. 2015 May 18;10(5):e0126556. doi: 10.1371/journal.pone.0126556. eCollection 2015.

Backbone

• Vector backbone: pRSET
• Backbone size w/o insert (bp): 2807
• Total vector size (bp): 3800
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VWF A2 domain aa 1594-1670_TEV_Venus_7X His
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 989
• GenBank ID: NM_000552.3| DQ092360.1
• Entrez Gene: VWF (a.k.a. F8VWF, VWD)
• Promoter: T7 Promoter
• Tags / Fusion Proteins:
  • 7X His (C terminal on insert)
  • Venus (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7 Promoter
• 3′ sequencing primer: T7 Terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry