pcDNA3-ER-GCaMP3
(Plasmid #64854)

• Purpose: Endoplasmic reticulum (ER)-targeted version of the Ca2+ indicator GCaMP3; targeted to cytosolic face of ER membrane.
• Depositing Lab: Jin Zhang
• Publication: Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.7554/eLife.03765.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5446
• Total vector size (bp): 5980
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CYP450-GCaMP3
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1440
• Promoter: CMV
• Tag / Fusion Protein:
  • N-terminal 27 amino acids from CYP450 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA3-ER-GCaMP3
• A portion of this plasmid was derived from a plasmid made by: The original GCaMP3 construct (Addgene 22692) was developed by Loren Looger’s lab.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry