pcDNA3.1(+)-Mito-CaNARi
(Plasmid
#64851)
-
PurposeMitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64851 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepcDNA3.1+
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 8452
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCaNARi
-
SpeciesH. sapiens (human), Synthetic
-
Insert Size (bp)3108
- Promoter CMV
-
Tags
/ Fusion Proteins
- Cerulean (N terminal on insert)
- Venus (C terminal on insert)
- N-terminal 30 amino acids from DAKAP1 (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7 forward
- 3′ sequencing primer BGH reverse (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA3.1(+)-Mito-CaNARi was a gift from Jin Zhang (Addgene plasmid # 64851 ; http://n2t.net/addgene:64851 ; RRID:Addgene_64851) -
For your References section:
Calmodulin-controlled spatial decoding of oscillatory Ca2+ signals by calcineurin. Mehta S, Aye-Han NN, Ganesan A, Oldach L, Gorshkov K, Zhang J. eLife. 2014 Jul 23;3:e03765. doi: 10.7554/eLife.03765. 10.7554/eLife.03765 PubMed 25056880
