pcDNA3.1(+)-PM-CaNARi
(Plasmid #64850)

• Purpose: Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) activation.
• Depositing Lab: Jin Zhang
• Publication: Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.7554/eLife.03765.

Backbone

• Vector backbone: pcDNA3.1+
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 8395
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lyn-CaNARi
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 3051
• Promoter: CMV
• Tags / Fusion Proteins:
  • Cerulean (N terminal on insert)
  • Venus (C terminal on insert)
  • N-terminal targeting sequence from Lyn kinase (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA3.1(+)-PM-CaNARi

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry