pRSET-XS-VWF
(Plasmid #64847)

• Purpose: Expresses aa1594–1670 of VWF A2 domain flanked by Venus and Cerulean and tagged with 7X His at C terminus
• Depositing Lab: Sriram Neelamegham
• Publication: Dayananda et al Anal Biochem. 2011 Mar 15;410(2):206-13. doi: 10.1016/j.ab.2010.12.005. Epub 2010 Dec 10.

Backbone

• Vector backbone: pRSET
• Backbone size w/o insert (bp): 2807
• Total vector size (bp): 4520
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Venus_VWF A2 domain aa 1594-1670_Cerulean_TEV_7X His
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1713
• GenBank ID: NM_000552.3| DQ092360.1
• Entrez Gene: VWF (a.k.a. F8VWF, VWD)
• Promoter: T7
• Tags / Fusion Proteins:
  • 7X His (C terminal on insert)
  • Venus (N terminal on insert)
  • Cerulean (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 Terminator

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry