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Addgene

pQE-60 Grx1-roGFP2-His
(Plasmid #64799)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64799 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQE-60
  • Backbone manufacturer
    Qiagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Glutaredoxin-1
  • Alt name
    GLRX1_HUMAN
  • Species
    H. sapiens (human)
  • Entrez Gene
    GLRX (a.k.a. GRX, GRX1)
  • Tags / Fusion Proteins
    • roGFP2 (C terminal on insert)
    • His (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Discrepancies between the QC and full sequence have no functional consequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQE-60 Grx1-roGFP2-His was a gift from Tobias Dick (Addgene plasmid # 64799 ; http://n2t.net/addgene:64799 ; RRID:Addgene_64799)

  • For your References section:

    Real-time imaging of the intracellular glutathione redox potential. Gutscher M, Pauleau AL, Marty L, Brach T, Wabnitz GH, Samstag Y, Meyer AJ, Dick TP. Nat Methods. 2008 Jun;5(6):553-9. doi: 10.1038/nmeth.1212. Epub 2008 May 11. 10.1038/nmeth.1212 PubMed 18469822
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