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pGL3-IRES-Lin28b-P3
(Plasmid #64794)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64794 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3-Basic-IRES
  • Backbone manufacturer
    Joshua Mendell
  • Backbone size w/o insert (bp) 5408
  • Total vector size (bp) 6561
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl2
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lin28b promoter P3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1153
  • Entrez Gene
    LIN28B (a.k.a. CSDD2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer GLprimer2
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-IRES-Lin28b-P3 was a gift from Joshua Mendell (Addgene plasmid # 64794 ; http://n2t.net/addgene:64794 ; RRID:Addgene_64794)

  • For your References section:

    Lin-28B transactivation is necessary for Myc-mediated let-7 repression and proliferation. Chang TC, Zeitels LR, Hwang HW, Chivukula RR, Wentzel EA, Dews M, Jung J, Gao P, Dang CV, Beer MA, Thomas-Tikhonenko A, Mendell JT. Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3384-9. doi: 10.1073/pnas.0808300106. Epub 2009 Feb 11. 10.1073/pnas.0808300106 PubMed 19211792
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