Skip to main content
Addgene

pGL3-IRES-miR34a-P3
(Plasmid #64787)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64787 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGL3-Basic-IRES
  • Backbone manufacturer
    Joshua Mendell
  • Backbone size w/o insert (bp) 5408
  • Total vector size (bp) 6871
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    miR-34a promoter P3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1463
  • Entrez Gene
    MIR34A (a.k.a. MIRN34A, miRNA34A, mir-34, mir-34a)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer GLprimer2
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-IRES-miR34a-P3 was a gift from Joshua Mendell (Addgene plasmid # 64787 ; http://n2t.net/addgene:64787 ; RRID:Addgene_64787)

  • For your References section:

    Transactivation of miR-34a by p53 broadly influences gene expression and promotes apoptosis. Chang TC, Wentzel EA, Kent OA, Ramachandran K, Mullendore M, Lee KH, Feldmann G, Yamakuchi M, Ferlito M, Lowenstein CJ, Arking DE, Beer MA, Maitra A, Mendell JT. Mol Cell. 2007 Jun 8;26(5):745-52. Epub 2007 May 31. 10.1016/j.molcel.2007.05.010 PubMed 17540599
Related items: