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pcDNA5FRT/Flag-FLJ10081
(Plasmid #64775)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64775 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5FRT
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 5070
  • Total vector size (bp) 7707
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLJ10081
  • Alt name
    NSL3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2637
  • GenBank ID
    BC063792
  • Entrez Gene
    KANSL3 (a.k.a. KIAA1310, NSL3, Rcd1)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5FRT/Flag-FLJ10081 was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 64775 ; http://n2t.net/addgene:64775 ; RRID:Addgene_64775)

  • For your References section:

    Subunit composition and substrate specificity of a MOF-containing histone acetyltransferase distinct from the male-specific lethal (MSL) complex. Cai Y, Jin J, Swanson SK, Cole MD, Choi SH, Florens L, Washburn MP, Conaway JW, Conaway RC. J Biol Chem. 2010 Feb 12. 285(7):4268-72. 10.1074/jbc.C109.087981 PubMed 20018852
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