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pET41s-GST-ATF6a-AD
(Plasmid #64774)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64774 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET41s
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5900
  • Total vector size (bp) 6350
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATF6a-AD (1-150)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    450
  • Mutation
    C-term deletion
  • GenBank ID
    BAA34722.1
  • Entrez Gene
    ATF6 (a.k.a. ACHM7, ATF6A, ATP6alpha)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET41s-GST-ATF6a-AD was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 64774 ; http://n2t.net/addgene:64774 ; RRID:Addgene_64774)

  • For your References section:

    Endoplasmic reticulum stress-responsive transcription factor ATF6alpha directs recruitment of the Mediator of RNA polymerase II transcription and multiple histone acetyltransferase complexes. Sela D, Chen L, Martin-Brown S, Washburn MP, Florens L, Conaway JW, Conaway RC. J Biol Chem. 2012 Jun 29;287(27):23035-45. doi: 10.1074/jbc.M112.369504. Epub 2012 May 10. 10.1074/jbc.M112.369504 PubMed 22577136
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