pAS
(Plasmid
#64746)
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PurposeYeast expression plasmid: ACC1 (ser1157ala)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 64746 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSP-GM2 (derived from pESC-URA)
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAS was a gift from Jens Nielsen (Addgene plasmid # 64746 ; http://n2t.net/addgene:64746 ; RRID:Addgene_64746) -
For your References section:
Improving production of malonyl coenzyme A-derived metabolites by abolishing Snf1-dependent regulation of Acc1. Shi S, Chen Y, Siewers V, Nielsen J. MBio. 2014 May 6;5(3):e01130-14. doi: 10.1128/mBio.01130-14. 10.1128/mBio.01130-14 PubMed 24803522