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Addgene

pAW
(Plasmid #64745)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64745 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP-GM2 (derived from pESC-URA)
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ACC1

Resource Information

  • Supplemental Documents

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Discrepancies between QC and full sequence have no functional consequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAW was a gift from Jens Nielsen (Addgene plasmid # 64745 ; http://n2t.net/addgene:64745 ; RRID:Addgene_64745)

  • For your References section:

    Improving production of malonyl coenzyme A-derived metabolites by abolishing Snf1-dependent regulation of Acc1. Shi S, Chen Y, Siewers V, Nielsen J. MBio. 2014 May 6;5(3):e01130-14. doi: 10.1128/mBio.01130-14. 10.1128/mBio.01130-14 PubMed 24803522
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