pKK01
(Plasmid
#64744)
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PurposeYeast expression plasmid: phaA, phaB, phaC
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 64744 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSP-GM2 (derived from pESC-URA)
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKK01 was a gift from Jens Nielsen (Addgene plasmid # 64744 ; http://n2t.net/addgene:64744 ; RRID:Addgene_64744) -
For your References section:
Engineering of acetyl-CoA metabolism for the improved production of polyhydroxybutyrate in Saccharomyces cerevisiae. Kocharin K, Chen Y, Siewers V, Nielsen J. AMB Express. 2012 Sep 25;2(1):52. doi: 10.1186/2191-0855-2-52. 10.1186/2191-0855-2-52 PubMed 23009357