pIYC08
(Plasmid
#64743)
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PurposeYeast expression plasmid: acsL641P, ALD6, ADH2, ERG10
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 64743 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepIYC04
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Vector typeYeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIYC08 was a gift from Jens Nielsen (Addgene plasmid # 64743 ; http://n2t.net/addgene:64743 ; RRID:Addgene_64743) -
For your References section:
Establishing a platform cell factory through engineering of yeast acetyl-CoA metabolism. Chen Y, Daviet L, Schalk M, Siewers V, Nielsen J. Metab Eng. 2013 Jan;15:48-54. doi: 10.1016/j.ymben.2012.11.002. Epub 2012 Nov 17. 10.1016/j.ymben.2012.11.002 PubMed 23164578