pEGFP-C1-PKM2
(Plasmid #64698)

• Purpose: Expression of GFP tagged PKM2 in mammalian cells
• Depositing Lab: Axel Ullrich
• Publication: Stetak et al Cancer Res. 2007 Feb 15;67(4):1602-8.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 6310
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PKM2
• Alt name: PKM transcript variant 1
• Species: H. sapiens (human)
• Insert Size (bp): 1610
• GenBank ID: NM_002654
• Entrez Gene: PKM (a.k.a. CTHBP, HEL-S-30, OIP3, PK3, PKM2, TCB, THBP1, p58)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (destroyed during cloning)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: GATCACATGGTCCTGCTGGAG
• 3′ sequencing primer: TGCATTCATTTTATGTTTCAGGTTC

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry