Skip to main content
Addgene

pFGET19_Ulp1
(Plasmid #64697)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64697 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET-28b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5368
  • Total vector size (bp) 5979
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ULP1
  • Alt name
    SUMO protease ULP1
  • Alt name
    NIB1
  • Species
    S. cerevisiae (budding yeast); Saccharomyces cerevisiae
  • Mutation
    Ulp1(403-621)
  • GenBank ID
    YPL020CNIB1 http://www.ncbi.nlm.nih.gov/gene/856087
  • Entrez Gene
    ULP1 (a.k.a. YPL020C, NIB1)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFGET19_Ulp1 was a gift from Hideo Iwai (Addgene plasmid # 64697 ; http://n2t.net/addgene:64697 ; RRID:Addgene_64697)

  • For your References section:

    Tandem SUMO fusion vectors for improving soluble protein expression and purification. Guerrero F, Ciragan A, Iwai H. Protein Expr Purif. 2015 Aug 20. pii: S1046-5928(15)30039-5. doi: 10.1016/j.pep.2015.08.019. 10.1016/j.pep.2015.08.019 PubMed 26297996

Ready-to-use antibodies

Looking for antibodies related to this plasmid? Check out these options:

Learn More
Related items:
Commonly requested with: