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Purpose(Empty Backbone) His-Smt3-NpuDnaEN102-CBD
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64696 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHYRSF1
- Backbone size (bp) 4100
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Modifications to backboneSpeI site introduced. CBD in EcoRI-HindIII. Smt3 into SpeI-NdeI.
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Vector typeBacterial Expression
- Promoter T7
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Tags
/ Fusion Proteins
- HisTag (N terminal on backbone)
- CBD (C terminal on backbone)
- NpuDnaE-N102 (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T7 term (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHYRSF53 was a gift from Hideo Iwai (Addgene plasmid # 64696 ; http://n2t.net/addgene:64696 ; RRID:Addgene_64696) -
For your References section:
Tandem SUMO fusion vectors for improving soluble protein expression and purification. Guerrero F, Ciragan A, Iwai H. Protein Expr Purif. 2015 Aug 20. pii: S1046-5928(15)30039-5. doi: 10.1016/j.pep.2015.08.019. 10.1016/j.pep.2015.08.019 PubMed 26297996
Map uploaded by the depositor.
