pLJSRSF3
(Plasmid #64692)

• Purpose: (Empty Backbone) His-GST-Smt3-NpuDnaEN102-CBD
• Depositing Lab: Hideo Iwai
• Publication: Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1046-5928(15)30039-5. doi: 10.1016/j.pep.2015.08.019.

Backbone

• Vector backbone: pHYRSF53
• Backbone size (bp): 4540
• Modifications to backbone: Addition of GST
• Vector type: Bacterial Expression
• Promoter: T7
• Tags / Fusion Proteins:
  • HisTag (N terminal on backbone)
  • GST (N terminal on insert)
  • Smt3 (N terminal on insert)
  • NpuDnaE-N102 (C terminal on backbone)
  • CBD (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 term

Resource Information

• Supplemental Documents:
  • pLJSRSF3.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry