TUBB5-Halo
(Plasmid #64691)

• Purpose: Expresses beta-tubulin labeled with Halo-tag
• Depositing Lab: Yasushi Okada
• Publication: Uno et al Nat Chem. 2014 Aug;6(8):681-9. doi: 10.1038/nchem.2002. Epub 2014 Jul 20.

Backbone

• Vector backbone: pHalo-N1
• Backbone manufacturer: We constructed pHalo-N1 by replacing EGFP of pEGFP-N1 with Halo-tag.
• Backbone size w/o insert (bp): 4900
• Total vector size (bp): 6200
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TUBB
• Alt name: TBB5
• Alt name: beta-tubulin
• Alt name: tubulin, beta
• Species: M. musculus (mouse)
• Insert Size (bp): 1332
• GenBank ID:
• Tag / Fusion Protein:
  • Halo-tag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Eco RI (not destroyed)
• 3′ cloning site: Sal I (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: EBV Reverse

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry