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p3xFLAG-CMV-RNF8-N-terminus(1-140)
(Plasmid #64675)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64675 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3XFLAG-CMV
  • Backbone manufacturer
    Sigma aldrich
  • Backbone size w/o insert (bp) 6400
  • Total vector size (bp) 6820
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ring finger protein 8
  • Alt name
    RNF8/N-terminus amino acids 1-140
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    420
  • Mutation
    only N terminus amino acids 1-140
  • GenBank ID
    NM_003958.3
  • Entrez Gene
    RNF8 (a.k.a. hRNF8)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer CMV-24 reverse: 5’-TATTAGGACAAGGCTGGTGGGCAC-3’
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3xFLAG-CMV-RNF8-N-terminus(1-140) was a gift from Marianne Farnebo (Addgene plasmid # 64675 ; http://n2t.net/addgene:64675 ; RRID:Addgene_64675)

  • For your References section:

    The scaffold protein WRAP53beta orchestrates the ubiquitin response critical for DNA double-strand break repair. Henriksson S, Rassoolzadeh H, Hedstrom E, Coucoravas C, Julner A, Goldstein M, Imreh G, Zhivotovsky B, Kastan MB, Helleday T, Farnebo M. Genes Dev. 2014 Dec 15;28(24):2726-38. doi: 10.1101/gad.246546.114. 10.1101/gad.246546.114 PubMed 25512560
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