pEGFP-RNF8-Middle-part
(Plasmid
#64672)
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PurposeExpresses only Middle part of RNF8, lacking N and C terminus, amino acids 112-401
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 64672 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerclontech
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 5570
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namering finger protein 8
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Alt nameRNF8/Middle part amino acids 112-401
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SpeciesH. sapiens (human)
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Insert Size (bp)870
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MutationContaining only middle part of RNF8 amino acids 112-401
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GenBank IDNM_003958.3
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Entrez GeneRNF8 (a.k.a. hRNF8)
- Promoter CMV
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer pEGFPC1-for 5´-GATCACTCTCGGCATGGAC-3´
- 3′ sequencing primer pEGFPC1-rev 5´-CATTTTATGTTTCAGGTTCAGGG-3’ (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-RNF8-Middle-part was a gift from Marianne Farnebo (Addgene plasmid # 64672 ; http://n2t.net/addgene:64672 ; RRID:Addgene_64672) -
For your References section:
The scaffold protein WRAP53beta orchestrates the ubiquitin response critical for DNA double-strand break repair. Henriksson S, Rassoolzadeh H, Hedstrom E, Coucoravas C, Julner A, Goldstein M, Imreh G, Zhivotovsky B, Kastan MB, Helleday T, Farnebo M. Genes Dev. 2014 Dec 15;28(24):2726-38. doi: 10.1101/gad.246546.114. 10.1101/gad.246546.114 PubMed 25512560
Map uploaded by the depositor.