4RG2
(Plasmid
#64658)
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PurposeBacterial expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64658 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28-MHL
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Backbone manufacturerStructural Genomics Consortium (Addgene Plasmid #26096)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert name53BP1
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Alt namePDB IDs: 4RG2, 6UPT, 6VA5, 8EOM, 8F0W, 8SWJ
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Alt name53BP1:APC056-E04:C32069
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SpeciesH. sapiens (human)
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Insert Size (bp)372
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MutationSee comments
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Entrez GeneTP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)
- Promoter T7
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Tags
/ Fusion Proteins
- 6xHis-TEV (N terminal on backbone)
- MHHHHHHSSGRENLYFQG (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7 Terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
4RG2 was a gift from Cheryl Arrowsmith (Addgene plasmid # 64658 ; http://n2t.net/addgene:64658 ; RRID:Addgene_64658)