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Addgene

4RG1
(Plasmid #64657)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 64657 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pET28-MHL
  • Backbone manufacturer
    Structural Genomics Consortium (Addgene Plasmid #26096)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    C9orf114:JMC099-C04:C232626
  • Alt name
    C9orf114
  • Species
    H. sapiens (human)
  • Mutation
    See comments
  • Entrez Gene
    SPOUT1 (a.k.a. C9orf114, CENP-32, HSPC109)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer T7
  • 3′ sequencing primer sacBpro-R (agtgaacggcaggtatatgtg)
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    4RG1 was a gift from Cheryl Arrowsmith (Addgene plasmid # 64657 ; http://n2t.net/addgene:64657 ; RRID:Addgene_64657)
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