AR-V7-pcw107
(Plasmid #64635)

• Purpose: when used to produce lentivirus, express physiological levels of insert
• Depositing Labs: David Sabatini, Kris Wood
• Publication: Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. doi: 10.1126/scisignal.aaa1877.

Backbone

• Vector backbone: pcw107 (V5)
• Backbone manufacturer: John Doench/Kathleen Ottina
• Backbone size w/o insert (bp): 9240
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AR (transcript variant 1, splice isoform)
• Alt name: NM_000044.3
• Species: H. sapiens (human)
• Insert Size (bp): 1932
• Mutation: splice isoform*
• Entrez Gene: AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
• Promoter: PGK

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: hPGK-F
• 3′ sequencing primer: WPRE-R

Resource Information

• Supplemental Documents:
  • pCW107 Destseq-V5
• A portion of this plasmid was derived from a plasmid made by: Charles Sawyers Lab, MSK/HHMI
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

GCTG (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-P1-STOP

*Addgene sequence shows that there is an L57Q mutation in AR-V7 relative to NCBI sequences. There is also a "QQQ" insertion and a "GGGG" deletion relative to NM_000044.3. These mutations were in the original Sawyers lab clone and are not known to affect function.