Notch3 intracellular domain-pcw107-V5
(Plasmid #64623)

• Purpose: when used to produce lentivirus, express physiological levels of insert
• Depositing Labs: David Sabatini, Kris Wood
• Publication: Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. doi: 10.1126/scisignal.aaa1877.

Backbone

• Vector backbone: pcw107 (V5)
• Backbone manufacturer: John Doench/Kathleen Ottina
• Backbone size w/o insert (bp): 9240
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: NOTCH3
• Alt name: NM_000435.2
• Species: H. sapiens (human)
• Insert Size (bp): 1980
• Mutation: intracellular domain *
• Entrez Gene: NOTCH3 (a.k.a. CADASIL, CADASIL1, CASIL, IMF2, LMNS)
• Promoter: PGK
• Tag / Fusion Protein:
  • V5 (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: hPGK-F
• 3′ sequencing primer: WPRE-R

Resource Information

• Supplemental Documents:
  • pCW107 Destseq-V5
• A portion of this plasmid was derived from a plasmid made by: Nicholas Gaiano (Addgene plasmid # 26894)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

CCAG (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-H2-V5

*Addgene sequence shows that there is an A1757T mutation in Notch3 relative to NCBI sequences. This mutation was in the original Gaiano lab clone and is not known to affect function.