beta-catenin (S33A, S37A, T41A, S45A)-pcw107-V5
(Plasmid #64613)

• Purpose: when used to produce lentivirus, express physiological levels of insert
• Depositing Labs: David Sabatini, Kris Wood
• Publication: Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. doi: 10.1126/scisignal.aaa1877.

Backbone

• Vector backbone: pcw107 (V5)
• Backbone manufacturer: John Doench/Kathleen Ottina
• Backbone size w/o insert (bp): 9240
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Ctnnb1
• Alt name: NM_001165902.1
• Species: M. musculus (mouse)
• Insert Size (bp): 2346
• Mutation: S33A, S37A, T41A, S45A
• Entrez Gene: Ctnnb1 (a.k.a. Bfc, Catnb, Mesc)
• Promoter: PGK
• Tag / Fusion Protein:
  • V5 (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: hPGK-F
• 3′ sequencing primer: WPRE-R

Resource Information

• Supplemental Documents:
  • pCW107 Destseq-V5
• A portion of this plasmid was derived from a plasmid made by: Tannishtha Reya (Addgene plasmid # 14717)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

AAAT (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-E1-V5