IKKalpha (S176E,S180E)-pcw107-V5
(Plasmid #64608)

• Purpose: when used to produce lentivirus, express physiological levels of insert
• Depositing Labs: David Sabatini, Kris Wood
• Publication: Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. doi: 10.1126/scisignal.aaa1877.

Backbone

• Vector backbone: pcw107 (V5)
• Backbone manufacturer: John Doench/Kathleen Ottina
• Backbone size w/o insert (bp): 9240
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CHUK
• Alt name: NM_001278.3
• Species: H. sapiens (human)
• Insert Size (bp): 2238
• Mutation: S176E, S180E
• Entrez Gene: CHUK (a.k.a. BPS2, IKBKA, IKK-1, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16)
• Promoter: PGK
• Tag / Fusion Protein:
  • V5 (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: hPGK-F
• 3′ sequencing primer: WPRE-R

Resource Information

• Supplemental Documents:
  • pCW107 Destseq-V5
• A portion of this plasmid was derived from a plasmid made by: Michael Karin Lab, UCSD

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

CCGG (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-C1-V5

Addgene NGS results found a V246A variant in the IKK-alpha open reading frame. This variant was present in the published plasmid and will function as described.