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Addgene

pcDNA3-TfR-OVA
(Plasmid #64600)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64600 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Total vector size (bp) 6640
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ovalbumin
  • Species
    G. gallus (chicken)
  • Promoter CMV
  • Tag / Fusion Protein
    • Transmembrane domain of Transferrin Receptor (TfR) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-TfR-OVA was a gift from Sandra Diebold & Martin Zenke (Addgene plasmid # 64600 ; http://n2t.net/addgene:64600 ; RRID:Addgene_64600)

  • For your References section:

    MHC class II presentation of endogenously expressed antigens by transfected dendritic cells. Diebold SS, Cotten M, Koch N, Zenke M. Gene Ther. 2001 Mar;8(6):487-93. 10.1038/sj.gt.3301433 PubMed 11313828
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