pcDNA3-Ii-OVA.II
(Plasmid #64597)

• Purpose: Expression of invariant chain fused to chicken ovalbumin 315-353 amino acids as model antigen for study of MHC class II presentation
• Depositing Labs: Sandra Diebold, Martin Zenke
• Publication: Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Total vector size (bp): 6827
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Ovalbumin
• Species: G. gallus (chicken)
• Mutation: Only amino acids 315-353 (class II epitopes)
• Promoter: CMV
• Tag / Fusion Protein:
  • Invariant chain (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry