pcDNA3-deltaOVA
(Plasmid #64595)

• Purpose: Expression of N-terminally truncated chicken ovalbumin as model antigen for study of MHC class I and class II presentation
• Depositing Labs: Sandra Diebold, Martin Zenke
• Publication: Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Total vector size (bp): 6231
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Ovalbumin
• Species: G. gallus (chicken)
• Mutation: Lacking first 49 aa (leader sequence for translocation into the ER)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HpaI (destroyed during cloning)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry