pmax pona 12xTRICK 24xMS2SL Notes

This gel depicts the NEB 1kb DNA ladder and a digestion of the plasmid 64542 with XhoI and StuI.

The expected pattern of bands was observed.

This gel depicts the NEB 1kb DNA ladder and a digestion of plasmid 64542 with XhoI and StuI.

We expected the following banding patterns:

XhoI single digest: 1 band @ 7.2kb

StuI single digest: 1 band @ 7.2kb

XhoI / StuI double digest: 2 bands at 5.6kb and 1.6 kb.

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pmax pona 12xTRICK 24xMS2SL with the following enzymes: NheI-HF and HpaI.

We predict the following pattern:

Nhe - 7.2kb
HpaI - 7.2kb
Nhe + HpaI - 3.9kb & 3.3kb

We see the expected result.

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pmax pona 12xTRICK 24xMS2SL with the following enzymes: BamHI-HF and NheI-HF.

Expected Bands
BamHI-HF/ NheI-HF: 5.3 kb and 1.8 kb.

We observe the expected bands for this digest.