FRP1671
(Plasmid #64538)

• Purpose: Inducible MET15 gene
• Depositing Lab: Joerg Stelling
• Publication: Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144.

Backbone

• Vector backbone: pRG216
• Backbone manufacturer: Robert Gnuegge
• Backbone size w/o insert (bp): 4557
• Total vector size (bp): 6727
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MET15
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 2266
• Entrez Gene: MET17 (a.k.a. YLR303W, MET15, MET25)
• Promoter: (lexA-box)_4-P_CYC1_minimal

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The insert's promoter was derived from FRP793 (Ottoz et al. [2014]: Inducible, tightly regulated and growth condition-independent transcription factor in Saccharomyces cerevisiae.).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry