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Addgene

FRP1625
(Plasmid #64537)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64537 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRG205
  • Backbone manufacturer
    Robert Gnuegge
  • Backbone size w/o insert (bp) 4646
  • Total vector size (bp) 7398
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LexA-ER-B42
  • Species
    Synthetic
  • Insert Size (bp)
    2848
  • Promoter ACT1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The insert was derived from FRP718 (Ottoz et al. [2014]: Inducible, tightly regulated and growth condition-independent transcription factor in Saccharomyces cerevisiae.).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FRP1625 was a gift from Joerg Stelling (Addgene plasmid # 64537 ; http://n2t.net/addgene:64537 ; RRID:Addgene_64537)

  • For your References section:

    A shuttle vector series for precise genetic engineering of Saccharomyces cerevisiae. Gnugge R, Liphardt T, Rudolf F. Yeast. 2015 Dec 9. doi: 10.1002/yea.3144. 10.1002/yea.3144 PubMed 26647923
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