pRG236
(Plasmid
#64534)
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Purpose(Empty Backbone) Integrative E. coli/S. cerevisiae shuttle vector
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64534 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRG236
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Backbone manufacturerRobert Gnuegge
- Backbone size (bp) 3763
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Promoter-truncated URA3d marker gene.
Integrates at Ty1 delta sites.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRG236 was a gift from Joerg Stelling (Addgene plasmid # 64534 ; http://n2t.net/addgene:64534 ; RRID:Addgene_64534) -
For your References section:
A shuttle vector series for precise genetic engineering of Saccharomyces cerevisiae. Gnugge R, Liphardt T, Rudolf F. Yeast. 2015 Dec 9. doi: 10.1002/yea.3144. 10.1002/yea.3144 PubMed 26647923
Map uploaded by the depositor.
