pRG223MX
(Plasmid
#64523)
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Purpose(Empty Backbone) Episomal E. coli/S. cerevisiae shuttle vector
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64523 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRG223MX
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Backbone manufacturerRobert Gnuegge
- Backbone size (bp) 5125
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Vector typeYeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe HIS3MX marker gene was derived from pFA6a-HIS3MX6 (Wach et al. [1997]: Heterologous HIS3 marker and GFP reporter modules for PCR-targeting in Saccharomyces cerevisiae.).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRG223MX was a gift from Joerg Stelling (Addgene plasmid # 64523 ; http://n2t.net/addgene:64523 ; RRID:Addgene_64523) -
For your References section:
A shuttle vector series for precise genetic engineering of Saccharomyces cerevisiae. Gnugge R, Liphardt T, Rudolf F. Yeast. 2015 Dec 9. doi: 10.1002/yea.3144. 10.1002/yea.3144 PubMed 26647923
Map uploaded by the depositor.
