pGL2-HKII-E
(Plasmid #64517)

• Purpose: AS-30D hepatoma hexokinase II 0.1 kbp promoter-luciferase reporter vector
• Depositing Lab: Peter Pedersen
• Publication: Mathupala et al J Biol Chem. 2001 Nov 16;276(46):43407-12. Epub 2001 Sep 13.

Backbone

• Vector backbone: pGL2-Basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5598
• Total vector size (bp): 6120
• Modifications to backbone: None
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): SURE
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Type II hexokinase gene, partial cds and promoter region
• Alt name: HKII
• Alt name: HK2
• Species: R. norvegicus (rat)
• Insert Size (bp): 523
• Mutation: Isolated from AS-30D rat hepatoma
• GenBank ID: U19605.2
• Entrez Gene: Hk2
• Promoter: Hexokinase II
• Tag / Fusion Protein:
  • firefly luciferase

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (destroyed during cloning)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: GL primer 1
• 3′ sequencing primer: GL primer 2

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For stability the plasmid is maintained in E. coli SURE. For reporter studies it is propagated in E. coli SCS110 or other methylation deficient strain.

Note: Addgene's quality control sequencing has identified a few single nucleotide discrepancies with the depositor's reference sequence. However, the plasmid accurately reflects the construct as it was used in the associated publication, and should function as described.