pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2
(Plasmid #64512)

• Purpose: Expression of St1 dCas9-3XTagBFP2 in mammalian cells
• Depositing Lab: Thoru Pederson
• Publication: Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23.

Backbone

• Vector backbone: pHAGE-DEST
• Backbone size w/o insert (bp): 6000
• Total vector size (bp): 12293
• Vector type: Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: St1 dCas9
• Species: Synthetic
• Insert Size (bp): 3357
• Promoter: CMV-TO
• Tag / Fusion Protein:
  • 3XmTagBFP2 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: cgaaggaatagaagaagaaggtgga
• 3′ sequencing primer: CCAAAGGGAGATCCGACTCG

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry