FRP1483
(Plasmid
#64511)
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PurposeExpresses destabilized Ametrine in yeast.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64511 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRG205
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Backbone manufacturerRobert Gnuegge
- Backbone size w/o insert (bp) 4646
- Total vector size (bp) 6553
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAmetrine-ADH1-tail
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SpeciesSynthetic
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Insert Size (bp)2003
- Promoter TDH3
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FRP1483 was a gift from Joerg Stelling (Addgene plasmid # 64511 ; http://n2t.net/addgene:64511 ; RRID:Addgene_64511) -
For your References section:
A shuttle vector series for precise genetic engineering of Saccharomyces cerevisiae. Gnugge R, Liphardt T, Rudolf F. Yeast. 2015 Dec 9. doi: 10.1002/yea.3144. 10.1002/yea.3144 PubMed 26647923
Map uploaded by the depositor.
