PSUC2-GFPantisense-CYC1t-pRS413
(Plasmid
#64404)
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PurposeSUC2 regulated expression of an antisense RNAi construct for GFP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64404 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS413
- Backbone size w/o insert (bp) 4970
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Vector typeYeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFPantisense
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Insert Size (bp)2219
- Promoter SUC2
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byEuroscarf
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PSUC2-GFPantisense-CYC1t-pRS413 was a gift from Claudia Vickers (Addgene plasmid # 64404 ; http://n2t.net/addgene:64404 ; RRID:Addgene_64404) -
For your References section:
Dynamic regulation of gene expression using sucrose responsive promoters and RNA interference in Saccharomyces cerevisiae. Williams TC, Espinosa MI, Nielsen LK, Vickers CE. Microb Cell Fact. 2015 Apr 1;14:43. doi: 10.1186/s12934-015-0223-7. 10.1186/s12934-015-0223-7 [pii] PubMed 25886317
